Tris buffer is widely used as a solvent for nucleic acids and proteins

Tris is a weak base with a pKa of 8.1 at room temperature (25 ° C); according to buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2.
The pH of the aqueous solution of the Tris base is about 10.5, and hydrochloric acid is usually added to adjust the pH to a desired value to obtain a buffer of the pH. But also pay attention to the effect of temperature on the pKa of Tris.
Tris buffers are not only widely used as solvents for nucleic acids and proteins, but also for many important uses. Tris is used for protein crystal growth under different pH conditions. The low ionic strength characteristics of Tris buffer can be used for the formation of intermediate fibers of C. elegans lamin. Tris is also one of the main components of protein electrophoresis buffers. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard.